Abstract: Overlying rock separation grouting technology is one of the key means to control surface subsidence and protect surface buildings and structures. Based on the 12070 working face of a certain mine of Yimei Group, and focuses on the problem of surface movement and deformation control in underground mining in villages. By comprehensively applying the means of “theoretical analysis – on-site monitoring – numerical simulation”, it systematically explores the movement law of overlying rocks and the effect of de-stratification grouting for settlement reduction in this working face. The distribution characteristics of the key layers of the overburden rock on the 12070 working face were clearly identified through the discrim-ination of key layers. Combined with 3DEC numerical simulation and probability integration method, the grouting layer location analysis was carried out. The development height range of the water-conducting fracture zone was 54.56−103.20 m, providing a basis for the selection of grouting layer locations. The results show that there are two key layers in the overburden rock of the 12070 working face, namely the fine-grained sandstone key layer 143.65 m away from the coal seam roof and the medium-grained sandstone sub-key layer 47.94 m away. After the stratification grouting of the overlying rock, the expected maximum surface subsidence was reduced to 268.3 mm, the maximum horizontal deformation was reduced to 1.9 mm/m, the surface subsidence reduction rate reached 77.6%, and the damage grades of the dense buildings and structures above the working face were all within the range of grade I damage, which can achieve the safe mining of coal resources under the dense buildings and structures and the coordinated protection of the surface environment. The research results can provide theoretical references and practical basis for the engineering application of stratified grouting and settlement reduction technology for dense buildings and structures under similar geological conditions.